Coding

Part:BBa_K1199046

Designed by: Tian Wang   Group: iGEM13_UESTC_Life   (2013-09-17)

DhaA31-P2A-HheCW249P 1,2,3-tricholoropropane(TCP)->Glycerol

P2A is sequence from foot-and-mouth disease virus. It links DhaA and HheC. When it is expressed in E.coli the P2A peptide links DhaA and HheC. And the chimeric protein also has the activity of DhaA and HheC.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 681
    Illegal NgoMIV site found at 807
    Illegal AgeI site found at 1600
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None